Abstract
The Candida albicans HOG1 gene (HOG1CA) was cloned by functional complementation of the osmosensitive phenotype associated with Saccharomyces cerevisiae hog1 delta mutants. HOG1CA codes for a 377-amino-acid protein, 78% identical to S. cerevisiae Hog1p. A C. albicans hog1 null mutant was found to be sensitive to osmotic stress and failed to accumulate glycerol on high-osmolarity media.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptation, Physiological / genetics
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Calcium-Calmodulin-Dependent Protein Kinases / genetics*
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Candida albicans / genetics*
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Cloning, Molecular
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Genes, Fungal*
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Genetic Complementation Test
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Glycerol / metabolism*
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Mitogen-Activated Protein Kinases*
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Molecular Sequence Data
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Mutagenesis, Insertional
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Osmotic Pressure
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Phenotype
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Saccharomyces cerevisiae Proteins*
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Sequence Analysis, DNA
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Signal Transduction / genetics
Substances
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Saccharomyces cerevisiae Proteins
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Calcium-Calmodulin-Dependent Protein Kinases
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HOG1 protein, S cerevisiae
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Mitogen-Activated Protein Kinases
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Glycerol