Cloning and sequencing of the novel abortive infection gene abiH of Lactococcus lactis ssp. lactis biovar. diacetylactis S94

FEMS Microbiol Lett. 1996 Sep 1;142(2-3):295-9. doi: 10.1111/j.1574-6968.1996.tb08446.x.

Abstract

A gene which encodes resistance by abortive infection (Abi+) to bacteriophage was cloned from Lactococcus lactis ssp. lactis biovar. diacetylactis S94. This gene was found to confer a reduction in efficiency of plating and plaque size for prolate-headed bacteriophage phi 53 (group I of homology) and total resistance to the small isometric-headed bacteriophage phi 59 (group III of homology). The cloned gene is predicted to encode a polypeptide of 346 amino acid residues with a deduced molecular mass of 41 455 Da. No homology with any previously described genes was found. A probe was used to determine the presence of this gene in two strains on 31 tested.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacteriophages / pathogenicity*
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • Immunity, Innate / genetics
  • Lactococcus lactis / genetics*
  • Lactococcus lactis / virology*
  • Molecular Sequence Data
  • Plasmids
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA

Substances

  • Bacterial Proteins

Associated data

  • GENBANK/X97651