Abstract
Spinal cord (SC) T cells were isolated at the onset of actively induced experimental autoimmune encephalomyelitis (EAE) and sorted for the presence of the OX-40 activation marker. Previously, we reported an enhanced bias in V beta 8.2 expression as well as enhanced proliferative responses to basic protein antigens among the OX-40+ SC T cells. Here we demonstrate that CDR3 motifs associated with EAE are present at a significantly higher frequency in V beta 8.2 sequences of OX-40+ SC T cells (16/17) compared with those of OX-40- SC T cells (5/17). Thus, the OX-40 antigen may be useful as a marker to isolate and characterize autoantigen-specific T cells from the site of inflammation in T-cell-mediated autoimmune diseases.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Surface / immunology
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Autoantigens / immunology
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Encephalomyelitis, Autoimmune, Experimental / immunology
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Encephalomyelitis, Autoimmune, Experimental / metabolism*
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Female
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Membrane Glycoproteins*
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Myelin Basic Protein / immunology
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Myelin Basic Protein / metabolism*
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Rats
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Rats, Inbred Lew
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Receptors, Antigen, T-Cell / immunology*
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Receptors, Tumor Necrosis Factor / immunology*
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Sequence Analysis, DNA
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Spinal Cord / cytology
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Spinal Cord / immunology
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Spinal Cord / metabolism*
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T-Lymphocytes / immunology
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T-Lymphocytes / metabolism*
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Tumor Necrosis Factors
Substances
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Antigens, Surface
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Autoantigens
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Membrane Glycoproteins
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Myelin Basic Protein
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Receptors, Antigen, T-Cell
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Receptors, Tumor Necrosis Factor
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Tnfsf4 protein, rat
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Tumor Necrosis Factors