Abstract
The phylogenetic compositions of bacterioplankton assemblages from San Francisco Bay and Tomales Bay, Calif., differed substantially when analyzed by PCR-denaturing gradient gel electrophoresis; these differences are consistent with the results of previous studies demonstrating differences in their metabolic capabilities. PCR-denaturing gradient gel electrophoresis analysis of complex microbial assemblages was sensitive and reliable, and the results were reproducible as shown by experiments with constructed and naturally occurring assemblages.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Bacteria / classification
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Bacteria / genetics*
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Bacteria / isolation & purification
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Base Sequence
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California
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DNA Primers / genetics
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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DNA, Ribosomal / genetics
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DNA, Ribosomal / isolation & purification
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Electrophoresis, Polyacrylamide Gel
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Molecular Sequence Data
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Phylogeny
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Plankton / classification
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Plankton / genetics*
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Plankton / microbiology*
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Polymerase Chain Reaction
Substances
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DNA Primers
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DNA, Bacterial
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DNA, Ribosomal