Eicosanoids are thought to play a role in the regulation of invertebrate reproduction, as they do in vertebrate systems. This was investigated using the previtellogenic ovary of the freshwater prawn Macrobrachium rosenbergii as a biological model. Concentrations of prostaglandin E2 (PGE2), assessed by means of radioimmunoassay, in the previtellogenic ovary (oocyte diameter 20-40 microns) were 32.4 +/- 14.1 pg/mg ovary. Preincubation of the ovary with indomethacin (10 microM) inhibited PGE2 synthesis by 43%. In addition, if indomethacin was added to the culture medium, cAMP levels decreased by 48%. When previtellogenic ovaries were incubated in vitro with PGE2 (0.05 micrograms/ml medium and up), cAMP levels in the tissue homogenate sharply increased. The levels of cAMP rose most significantly (up to 10-fold) when 1-10 micrograms PGE2/ml medium was applied. These results suggest that PGE2, and possibly other prostaglandins, may play a role in the endocrine regulation of crustacean reproduction.