Abstract
The promoter of TGF-beta type II receptor lacks TATA box or CAAT box. In order to define the exact transcriptional start site(s), we used "oligo-capping" method developed by ourselves. The major transcripts were started from GAA located between -35 and -33 relative to 5' end of cDNA. Other minor transcripts started from -4, +11 and +17. Deletion analysis of this promoter region revealed that it contained two adjacently located promoters (P1 and P2) capable of acting by itself. The P1 is located between -22 and +55 which covers the minor transcriptional start sites described above. The P2 is located just upstream of the P1 between -137 and -22. The results showed all of the transcripts were started either from P1 or P2.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Cell Line
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Cloning, Molecular
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Humans
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Luciferases / biosynthesis
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Oligodeoxyribonucleotides
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Promoter Regions, Genetic*
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Protein Serine-Threonine Kinases
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Receptor, Transforming Growth Factor-beta Type II
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Receptors, Transforming Growth Factor beta / biosynthesis*
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Receptors, Transforming Growth Factor beta / genetics*
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Recombinant Proteins / biosynthesis
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Sequence Deletion
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TATA Box
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Transcription, Genetic*
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Transfection
Substances
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Oligodeoxyribonucleotides
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RNA, Messenger
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Receptors, Transforming Growth Factor beta
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Recombinant Proteins
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Luciferases
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Protein Serine-Threonine Kinases
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Receptor, Transforming Growth Factor-beta Type II