Abstract
The Azotobacter vinelandii mod locus, which is involved in high-affinity molybdate transport and the early event in Mo metabolism, consists of two divergently transcribed operons, modG and modEABC. modA, modB and modC encode the components of the high-affinity molybdate transporter, and modG encodes a Mo-binding protein. High concentrations of Mo repressed transcription of both operons. The modEABC operon was also repressed by tungstate and to a lesser extent by vanadate. modE, the first gene in the modEABC operon, controlled the Mo-dependent transcription of both operons. It was not involved in the metal regulation of alternative nitrogenase gene expression. Although a modE mutant constitutively expressed genes encoding the molybdate transporter, it had a reduced rate of Mo accumulation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Azotobacter vinelandii / genetics
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Azotobacter vinelandii / metabolism*
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Bacterial Proteins*
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Base Sequence
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Carrier Proteins / biosynthesis*
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Cloning, Molecular
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DNA Primers
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Escherichia coli
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Escherichia coli Proteins*
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Gene Expression Regulation, Bacterial* / drug effects
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Genes, Bacterial
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Genotype
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Intracellular Signaling Peptides and Proteins
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Molecular Sequence Data
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Molybdenum / metabolism*
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Molybdenum / pharmacology
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Operon*
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Polymerase Chain Reaction
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Sequence Deletion
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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Bacterial Proteins
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Carrier Proteins
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DNA Primers
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Escherichia coli Proteins
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Intracellular Signaling Peptides and Proteins
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ModE protein, E coli
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ModE protein, bacteria
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ModG protein, Azotobacter vinelandii
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Transcription Factors
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Molybdenum