Changes in IgG mRNA half-life, transcription and nuclear and cytoplasmic abundance were studied in two cell lines which contain an identical Ig gamma 2a heavy chain but which differ in its expression. The A20.2J mouse lymphoma expresses about equal amounts of Ig gamma 2a secretory- and membrane-specific mRNAs whereas in the AXJ hybrids, resulting from the fusion of A20.2J with the J558L myeloma, the secretory-specific form dominates. Further evidence of dominance of the myeloma phenotype was seen in the large changes in mRNA abundance and nuclear accumulation as well as in a small increase in Ig gamma 2a mRNA half-lives for both secretory and membrane forms. Contributing to the observed > 100-fold increase in the ratio of secretory vs membrane forms of the Ig gamma 2a heavy chain in the AXJ hybrids are both a 10-fold decrease in the production of the membrane form by post-transcriptional RNA processing events and a approximately 6-7-fold decrease in the nuclear to cytoplasmic ratio for the Ig secretory gamma 2a and kappa light chain RNAs. Differential RNA accumulation in the nucleus in the lymphoma cell therefore contributes to the differential expression of Ig secretory mRNA.