Single-stranded DNA-binding protein enhances the stability of CTG triplet repeats in Escherichia coli

J Bacteriol. 1996 Aug;178(16):5042-4. doi: 10.1128/jb.178.16.5042-5044.1996.

Abstract

The stability of CTG triplet repeats was analyzed in Escherichia coli to identify processes responsible for their genetic instability. Using a biochemical assay for stability, we show that the absence of single-stranded-DNA-binding protein leads to an increase in the frequency of large deletions within the triplet repeats.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites
  • DNA-Binding Proteins / metabolism*
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism*
  • Humans
  • Myotonic Dystrophy / genetics
  • Plasmids*
  • Sequence Deletion
  • Trinucleotide Repeats*

Substances

  • DNA-Binding Proteins