Abstract
The stability of CTG triplet repeats was analyzed in Escherichia coli to identify processes responsible for their genetic instability. Using a biochemical assay for stability, we show that the absence of single-stranded-DNA-binding protein leads to an increase in the frequency of large deletions within the triplet repeats.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Binding Sites
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DNA-Binding Proteins / metabolism*
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Escherichia coli / genetics*
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Escherichia coli / metabolism*
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Humans
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Myotonic Dystrophy / genetics
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Plasmids*
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Sequence Deletion
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Trinucleotide Repeats*