A sequence-specific DNA-binding protein interacts with the xlnC upstream region of Streptomyces sp. strain EC3

FEMS Microbiol Lett. 1996 Aug 15;142(1):91-7. doi: 10.1111/j.1574-6968.1996.tb08413.x.

Abstract

The alignment of the promoter region of several Streptomyces xylanases shows three conserved sequences which could be involved in gene regulation. By electromobility shift assays these specific sequences, present only in Streptomyces xylanolytic strains, were identified as protein-binding sites. The sequence required for efficient recognition by the retarding protein appeared to be a 4-bp inverted repeat: 5'-CTTT-Nx-AAAG-3'. The DNA-protein affinity was influenced by the culture conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / metabolism*
  • Base Sequence
  • Binding Sites / genetics
  • DNA, Bacterial / genetics
  • DNA, Bacterial / metabolism
  • DNA-Binding Proteins / metabolism*
  • Genes, Bacterial
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Sequence Homology, Nucleic Acid
  • Streptomyces / genetics*
  • Streptomyces / metabolism*
  • Xylan Endo-1,3-beta-Xylosidase
  • Xylosidases / genetics

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Xylosidases
  • Xylan Endo-1,3-beta-Xylosidase

Associated data

  • GENBANK/M64552
  • GENBANK/M64553
  • GENBANK/U08894