A TLC scanning method for the determination of flavonol glycosides in the leaf of Ginkgo biloba has been established. The method includes hydrolysis of the flavonoids and subsequent quantitative TLC scanning assay of the aglycones obtained. Determinations were carried out with a Shimadzu CS-930 scanner, with lambda(S) = 370 nm and lambda(R) = 650 nm. The recoveries were 96.0%-99.6% with RSD of 1.03%-2.08%.