A transgenic mouse line with alpha-1,3/4-fucosyl-transferase cDNA: production and characteristics

Glycoconj J. 1995 Dec;12(6):795-801. doi: 10.1007/BF00731241.

Abstract

cDNA of human alpha-1,3/4-fucosyltransferase (Fuc-TIII) was placed under the control of the chicken beta-actin promoter and cytomegalovirus enhancer, then introduced into male pronuclei of fertilized mouse eggs. A transgenic mouse line thus obtained exhibited enhanced expression of Lex (4C9) antigen in endothelial cells located in the glomerulus, sinusoidal capillaries of the liver and capillaries of the heart. Furthermore, in the transgenic mice, sialyl dimeric Lex (FH6) and sialyl Lea (2D3) antigens were strongly expressed in the glomerular endothelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Carbohydrate Conformation
  • DNA, Complementary / biosynthesis*
  • DNA, Complementary / chemistry
  • Fucosyltransferases / genetics*
  • Kidney / immunology
  • Lewis X Antigen / genetics*
  • Liver / immunology
  • Lung / immunology
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Myocardium / immunology

Substances

  • DNA, Complementary
  • Lewis X Antigen
  • Fucosyltransferases
  • 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase