Toward fully automated high-resolution electron tomography

J C Fung; W Liu; W J de Ruijter; H Chen; C K Abbey; J W Sedat; D A Agard

doi:10.1006/jsbi.1996.0029

Toward fully automated high-resolution electron tomography

J Struct Biol. 1996 Jan-Feb;116(1):181-9. doi: 10.1006/jsbi.1996.0029.

Authors

J C Fung¹, W Liu, W J de Ruijter, H Chen, C K Abbey, J W Sedat, D A Agard

Affiliation

¹ Graduate Group in Biophysics, University of California at San Francisco 94143-0448, USA.

PMID: 8742742
DOI: 10.1006/jsbi.1996.0029

Abstract

Electron tomography is a powerful tool in elucidating the three-dimensional architecture of large biological complexes and subcellular organelles. Its use can be expanded through the simplification of the tomographic procedure by automation of its tasks. In this paper, we describe our EMACT/EMCAT system, which automates both tomographic data collection and reconstruction.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Automation
Centrosome / ultrastructure
Computer Graphics
Computer Simulation*
Microscopy, Electron*
Models, Structural*
Organelles / ultrastructure*
Software

Abstract

Publication types

MeSH terms

Grants and funding