Abstract
The complete amino acid sequences of the Lys-49 PLA2s from the venom of Deinagkistrodon acutus (from Taiwan and China) and Trimeresurus mucrosquamatus (Taiwan habu) were solved by a facile cDNA cloning and sequencing method. The deduced amino acid sequences of the Lys-49 PLA2s of both venoms are identical, suggesting close phylogenic relationship between this two snake species of different genera. In addition, by cloning and cDNA sequencing, the mRNA coding for a Arg-49 PLA2 homolog of low expression level was also found in the venom gland of T. mucrosquamatus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agkistrodon*
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Calcium / metabolism
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Crotalid Venoms / chemistry*
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Crotalid Venoms / enzymology
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Crotalid Venoms / genetics
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DNA, Complementary / chemistry
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DNA, Complementary / metabolism
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Lysine / chemistry
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Lysine / metabolism
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Molecular Sequence Data
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Phospholipases A / chemistry*
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Phospholipases A / genetics
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Phospholipases A / metabolism
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Phospholipases A2
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Species Specificity
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Trimeresurus*
Substances
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Crotalid Venoms
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DNA, Complementary
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Phospholipases A
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Phospholipases A2
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Lysine
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Calcium
Associated data
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GENBANK/X77647
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GENBANK/X77650
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GENBANK/X77651