LYS-49 phospholipase A2 homologs from venoms of Deinagkistrodon acutus and Trimeresurus mucrosquamatus have identical protein sequence

Toxicon. 1996 Apr;34(4):485-9. doi: 10.1016/0041-0101(95)00161-1.

Abstract

The complete amino acid sequences of the Lys-49 PLA2s from the venom of Deinagkistrodon acutus (from Taiwan and China) and Trimeresurus mucrosquamatus (Taiwan habu) were solved by a facile cDNA cloning and sequencing method. The deduced amino acid sequences of the Lys-49 PLA2s of both venoms are identical, suggesting close phylogenic relationship between this two snake species of different genera. In addition, by cloning and cDNA sequencing, the mRNA coding for a Arg-49 PLA2 homolog of low expression level was also found in the venom gland of T. mucrosquamatus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agkistrodon*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Calcium / metabolism
  • Crotalid Venoms / chemistry*
  • Crotalid Venoms / enzymology
  • Crotalid Venoms / genetics
  • DNA, Complementary / chemistry
  • DNA, Complementary / metabolism
  • Lysine / chemistry
  • Lysine / metabolism
  • Molecular Sequence Data
  • Phospholipases A / chemistry*
  • Phospholipases A / genetics
  • Phospholipases A / metabolism
  • Phospholipases A2
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Trimeresurus*

Substances

  • Crotalid Venoms
  • DNA, Complementary
  • Phospholipases A
  • Phospholipases A2
  • Lysine
  • Calcium

Associated data

  • GENBANK/X77647
  • GENBANK/X77650
  • GENBANK/X77651