Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay

J Clin Microbiol. 1996 Jun;34(6):1465-7. doi: 10.1128/jcm.34.6.1465-1467.1996.

Abstract

Conventional tube cell culture was compared with a 72-h, spin-amplified shell vial indirect immunofluorescence assay for the detection of enterovirus from clinical specimens. The sensitivity for the shell vial assay after resolution of discrepant results were 93 and 100%, respectively. The shell vial assay detected 93% of the positive cultures within 72 h of incubation while conventional tube culture detected only 51% of the positive cultures within the same time interval. The data suggest that a spin-amplified shell vial indirect immunofluorescence assay may be useful for the detection of enterovirus from clinical specimens.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Antibodies, Viral
  • Cells, Cultured
  • Cytopathogenic Effect, Viral
  • Diagnostic Errors
  • Enterovirus / classification
  • Enterovirus / immunology*
  • Enterovirus / isolation & purification*
  • Enterovirus Infections / diagnosis
  • Enterovirus Infections / virology
  • Evaluation Studies as Topic
  • Fluorescent Antibody Technique, Indirect* / statistics & numerical data
  • Humans
  • Macaca mulatta
  • Polymerase Chain Reaction / statistics & numerical data
  • Sensitivity and Specificity
  • Virology / methods*
  • Virology / standards
  • Virology / statistics & numerical data

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral