Abstract
Conventional tube cell culture was compared with a 72-h, spin-amplified shell vial indirect immunofluorescence assay for the detection of enterovirus from clinical specimens. The sensitivity for the shell vial assay after resolution of discrepant results were 93 and 100%, respectively. The shell vial assay detected 93% of the positive cultures within 72 h of incubation while conventional tube culture detected only 51% of the positive cultures within the same time interval. The data suggest that a spin-amplified shell vial indirect immunofluorescence assay may be useful for the detection of enterovirus from clinical specimens.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal*
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Antibodies, Viral
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Cells, Cultured
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Cytopathogenic Effect, Viral
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Diagnostic Errors
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Enterovirus / classification
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Enterovirus / immunology*
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Enterovirus / isolation & purification*
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Enterovirus Infections / diagnosis
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Enterovirus Infections / virology
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Evaluation Studies as Topic
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Fluorescent Antibody Technique, Indirect* / statistics & numerical data
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Humans
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Macaca mulatta
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Polymerase Chain Reaction / statistics & numerical data
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Sensitivity and Specificity
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Virology / methods*
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Virology / standards
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Virology / statistics & numerical data
Substances
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Antibodies, Monoclonal
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Antibodies, Viral