Evaluation of different absorbance ratios from infrared spectroscopy for analyzing the degree of deacetylation in chitin

Y Shigemasa; H Matsuura; H Sashiwa; H Saimoto

doi:10.1016/0141-8130(95)01079-3

Evaluation of different absorbance ratios from infrared spectroscopy for analyzing the degree of deacetylation in chitin

Int J Biol Macromol. 1996 Apr;18(3):237-42. doi: 10.1016/0141-8130(95)01079-3.

Authors

Y Shigemasa¹, H Matsuura, H Sashiwa, H Saimoto

Affiliation

¹ Department of Materials Science, Faculty of Engineering, Tottori University, Japan.

PMID: 8729036
DOI: 10.1016/0141-8130(95)01079-3

Abstract

Various acetylated chitosan derivatives and mixtures of chitin and chitosan, covering the range of the degree of deacetylation (DDA) from 0-100% were analyzed by 1H-NMR spectroscopy and infrared spectroscopy. The use of the 1070 cm-1 or 1030 cm-1 absorption band as an internal standard in the determination of DDA from the absorbance of the amide I bands at 1655 cm-1 and 1630 cm-1 or the amide II band at 1560 cm-1 was studied. There is a good correlation between the results from IR spectroscopy and those from 1H-NMR spectroscopy.

MeSH terms

Acetylation
Animals
Brachyura / chemistry
Carbohydrate Conformation
Carbohydrate Sequence
Chitin / analogs & derivatives*
Chitin / chemistry*
Chitosan
Evaluation Studies as Topic
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Spectrophotometry, Infrared

Substances

Chitin
Chitosan