Cigarette smoke-modified low density lipoprotein impairs endothelium-dependent relaxation in isolated rabbit arteries

Gen Pharmacol. 1996 Apr;27(3):477-81. doi: 10.1016/0306-3623(95)02074-8.

Abstract

1. We investigated the effects of cigarette smoke-modified low-density lipoprotein (CS-LDL) on endothelium-dependent relaxations in various isolated rabbit arteries, and compared them with those of oxidized LDL (Ox-LDL). 2. In aorta rings, acetylcholine-induced endothelium-dependent relaxations were attenuated by preincubation with CS-LDL and Ox-LDL. Endothelium-independent relaxations induced by sodium nitroprusside were not modified. Similar changes were observed in rings of coronary and basilar arteries. 3. These findings indicate that CS-LDL, as well as Ox-LDL, may decrease the release or activity of endothelium-derived relaxing factor in vascular endothelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / metabolism
  • Animals
  • Electrophoresis, Agar Gel
  • Endothelium, Vascular / physiology*
  • In Vitro Techniques
  • Lipoproteins, LDL / blood
  • Lipoproteins, LDL / chemistry
  • Lipoproteins, LDL / pharmacology*
  • Muscle Relaxation / drug effects
  • Muscle, Smooth, Vascular / drug effects*
  • Muscle, Smooth, Vascular / metabolism
  • Muscle, Smooth, Vascular / physiology
  • Nitric Oxide / metabolism
  • Nitroprusside / pharmacology
  • Oxidation-Reduction
  • Rabbits
  • Smoking / blood*
  • Thiobarbituric Acid Reactive Substances / chemistry
  • Vasodilator Agents / pharmacology

Substances

  • Lipoproteins, LDL
  • Thiobarbituric Acid Reactive Substances
  • Vasodilator Agents
  • Nitroprusside
  • Nitric Oxide
  • Acetylcholine