Abstract
A wheat cDNA encoding a glycine-rich RNA-binding protein, whGRP-1, was isolated. WhGRP-1 contains two conserved domains, the RNA-binding motif (RNP motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant RNA-binding proteins. Northern analysis revealed that whGRP-1 mRNA accumulates to high levels in roots and to lower levels in leaves of wheat seedlings, whGRP-1 mRNA accumulation is not enhanced by exogenous abscisic acid in seedlings and accumulates to very high levels during wheat embryo development, showing a pattern different from that of the ABA-inducible wheat Em gene.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Abscisic Acid / pharmacology
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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DNA, Complementary
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Gene Expression Regulation, Developmental / drug effects
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Gene Expression Regulation, Plant / drug effects
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Molecular Sequence Data
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Plant Proteins / genetics*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA-Binding Proteins / genetics*
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Triticum / embryology
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Triticum / genetics*
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Triticum / growth & development
Substances
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DNA, Complementary
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Plant Proteins
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RNA, Messenger
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RNA-Binding Proteins
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glycine-rich RNA-binding protein, plant
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Abscisic Acid