Physical mapping of a centromere-proximal region of chromosome IV-L defines the placement of genes USO1, MBP1, PSA1 and SLC1

D C Gardner; G C Tomlin; T Cele; G A Hamilton; C M James; L I Stateva; S G Oliver

doi:10.1002/(SICI)1097-0061(19960330)12:4%3C411::AID-YEA918%3E3.0.CO;2-8

Physical mapping of a centromere-proximal region of chromosome IV-L defines the placement of genes USO1, MBP1, PSA1 and SLC1

Yeast. 1996 Mar 30;12(4):411-3. doi: 10.1002/(SICI)1097-0061(19960330)12:4%3C411::AID-YEA918%3E3.0.CO;2-8.

Authors

D C Gardner¹, G C Tomlin, T Cele, G A Hamilton, C M James, L I Stateva, S G Oliver

Affiliation

¹ Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), U.K.

Abstract

A physical map of a 14.5 kb region close to the centromere on the left arm of chromosome IV of Saccharomyces cerevisiae is presented. This map has been constructed by restriction analysis of a clone from a YCp50 genomic library and by use of pre-existing and new sequence data from this region. The map reveals the following gene order (reading from the most centromere-distal to the most centromere-proximal locus): USO1/INT1-MBP1-PSA1-SLC1-YLA1 and defines the size of the open reading frames and intergenic regions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Centromere*
Chromosome Mapping
Chromosomes, Fungal*
Genes, Fungal*
Molecular Sequence Data
Saccharomyces cerevisiae / genetics*

Associated data

GENBANK/U19608