Abstract
Serotype b-specific polysaccharide antigen (SPA) was extracted from whole cells of Actinobacillus actinomycetemcomitans Y4 by autoclaving and purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300. SPA induced the release of monocyte and leukocyte chemotactic factors by human monocytes. Polymyxin B had almost no effect on the release of monocyte chemotactic factor, but a monoclonal antibody against SPA markedly inhibited it. Human monocytes stimulated with SPA exhibited the increased mRNA expression of monocyte chemoattractant protein 1 (MCP-1) and a neutrophil chemotactic factor, interleukin-8 (IL-8). On the other hand, SPA induced the release of IL-1, IL-6, and tumor necrosis factor (TNF) and enhanced the expression of IL-1alpha, IL-1beta, IL-6, and TNF alpha (TNF-alpha) mRNAs. Human monocytes expressed MCP-1 and IL-8 mRNAs when stimulated by human recombinant IL-1alpha, I1-1beta, IL-6, and TNF-alpha, suggesting that these inflammatory cytokines induced by SPA might participate in the production of chemotactic factors in human monocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aggregatibacter actinomycetemcomitans / classification
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Aggregatibacter actinomycetemcomitans / immunology*
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Antigens, Bacterial* / isolation & purification
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Antigens, Bacterial* / pharmacology
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Base Sequence
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Chemokine CCL2 / biosynthesis
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Chemokine CCL2 / genetics
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Chemotactic Factors / biosynthesis*
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Chemotactic Factors / genetics
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Cytokines / biosynthesis*
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Cytokines / genetics
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DNA Primers / genetics
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Gene Expression
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Humans
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In Vitro Techniques
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Interleukin-1 / biosynthesis
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Interleukin-1 / genetics
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Interleukin-6 / biosynthesis
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Interleukin-6 / genetics
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Interleukin-8 / biosynthesis
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Interleukin-8 / genetics
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Molecular Sequence Data
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Monocytes / immunology*
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Monocytes / metabolism
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Polysaccharides, Bacterial / immunology*
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Polysaccharides, Bacterial / isolation & purification
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Polysaccharides, Bacterial / pharmacology
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Serotyping
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Tumor Necrosis Factor-alpha / biosynthesis
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Tumor Necrosis Factor-alpha / genetics
Substances
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Antigens, Bacterial
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Chemokine CCL2
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Chemotactic Factors
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Cytokines
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DNA Primers
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Interleukin-1
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Interleukin-6
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Interleukin-8
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Polysaccharides, Bacterial
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RNA, Messenger
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Tumor Necrosis Factor-alpha