Abstract
The plant hormone auxin regulates various developmental processes including root formation, vascular development, and gravitropism. Mutations within the AUX1 gene confer an auxin-resistant root growth phenotype and abolish root gravitropic curvature. Polypeptide sequence similarity to amino acid permeases suggests that AUX1 mediates the transport of an amino acid-like signaling molecule. Indole-3-acetic acid, the major form of auxin in higher plants, is structurally similar to tryptophan and is a likely substrate for the AUX1 gene product. The cloned AUX1 gene can restore the auxin-responsiveness of transgenic aux1 roots. Spatially, AUX1 is expressed in root apical tissues that regulate root gravitropic curvature.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2,4-Dichlorophenoxyacetic Acid / pharmacology
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Amino Acid Sequence
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Amino Acid Transport Systems
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Amino Acids / metabolism
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Arabidopsis / chemistry
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Arabidopsis / genetics*
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Arabidopsis / growth & development
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Arabidopsis / metabolism
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Arabidopsis Proteins*
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Biological Transport
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Cloning, Molecular
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DNA, Bacterial / genetics
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Genes, Plant*
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Genetic Complementation Test
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Gravitropism*
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Indoleacetic Acids / metabolism
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Indoleacetic Acids / pharmacology
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Membrane Transport Proteins / chemistry
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Molecular Sequence Data
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Molecular Weight
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Mutation
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Plant Proteins / chemistry
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Plant Proteins / genetics*
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Plant Proteins / metabolism
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Plant Roots / growth & development*
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Plant Roots / metabolism
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Sequence Alignment
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Signal Transduction
Substances
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AUX1 protein, Arabidopsis
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Amino Acid Transport Systems
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Amino Acids
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Arabidopsis Proteins
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DNA, Bacterial
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Indoleacetic Acids
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Membrane Transport Proteins
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Plant Proteins
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T-DNA
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2,4-Dichlorophenoxyacetic Acid
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indoleacetic acid