Four thousand six hundred forty-five quarter milk samples from 1179 cows from 20 commercial dairy herds were examined in order to determine the prevalence of bacterial species. A total of 859 isolates from 839 (18.1%) culture positive samples could be assigned to 34 different species and subspecies. Diagnostics of staphylococcal species was based on conventional procedures able to differentiate between all 36 species and subspecies presently acknowledged. Staphylococcus aureus was found in 10.2% of the samples and was the most common species isolated. Streptococcus dysgalactiae (1.6%) and Streptococcus uberis (1.4%) were the second and third most common species isolated. Seventeen different coagulase negative staphylococcal species (CNS) were found in 4.1% of the samples. The most frequently isolated CNS were S. epidermidis (1.3%), S. chromogenes (1.0%) and S. simulans (0.7%). Isolates of S. aureus were phage typed, and isolates of S. epidermidis were investigated by phage typing, antibiogram typing, and biotyping. A total of 378 (79.9%) isolates of S. aureus could be typed by phages, assigning them to 18 different phage types. However, 6 phage types accounted for 92.1% of the typable isolates. One to 2 phage types predominated within each herd. Eleven (18%) isolates of S. epidermidis could be typed by phages, assigning the isolates to 3 different types. Biotyping of S. epidermidis produced a total of 8 different types, the most common accounting for 29.5% of the isolates. A total of 6 different antibiogram types were observed among all isolates of S. epidermidis. Resistance towards penicillin (36.1%), tetracycline (9.8%) and streptomycin (9.8%), were recorded in the isolates of S. epidermidis. However, 35 (57.4%) of the isolates were susceptible to all 12 antibiotics tested.
For at bestemme den relative fordeling af forskellige bakteriearter fra bovin mastitis blev 4645 kirtelprøver fra 20 danske malkekvægsbesætninger undersøgt. Fra 839 af kirtlerne blev der isoleret 859 frjrskcllige bak teri ei so later, som kunne inddeles i 34 forskellige bakteriearter og underarter. Stafylokok-diagnostikken var baseret på konventionelle procedurer som var i stand til at skeine mellem alle 36 anerkendte arter og underarter. Staphylococcus aureus blev isoleret fra 10,2% af kirtlerne og var den oftest isolerede patogen. Den næst mest almindelige patogen var Streptococcus dysgalactiae (1,6%) og trejde Streptococcus uteris (1,4%). Der blev isoleret 17 forskellige arter og underarter af koagulase negative stafylokokker. De mest almindelige var S. epidermidis (1,3%), S. chromogenes (1,0%) og S. simulans (0,7%). Isolateme af S. aureus blev fagtypet og isolater af S. epidermidis blev undersøgt for fagtypc, antibiogramtype og biotypc. lalt kunne 378 (79,9%) af S. aureus isolateme types med fager, som inddelte dem i 18 forskellige typer. Seks fagtyper udgjorde dog 92,1% af alle de typebare isolater En til 2 forskellige fagtyper dominerede indenfor hver besætning. Elleve (18%) af S. epidermidis isolateme kunne types med fager, som inddelte dem i 3 forskellige typer. Biotypning af S. epidermidis gav 8 forskellige typer, hvor den mest almindelige type udgjorde 29,5%. Seks forskellige antibiogramtyper forekom blandt alle isolater af S. epidermidis. Resistens mod penicillin (36,1%), tetracyclin (9,8%) og streptomycin (9,8%) blev observeret blandt S. epidermidis isolateme, Femogtredive (57,4%) af isolateme var dog følsomme over for alle 12 antibiotika, som der blev testet for.