An upstream positive regulatory element in human GM-CSF promoter is recognized by NF-kappa B/Rel family members

Biochem Biophys Res Commun. 1996 Jun 5;223(1):64-72. doi: 10.1006/bbrc.1996.0847.

Abstract

To further extend the previous analysis of cis-acting elements and cognate trans-acting factors that contribute to GM-CSF transcriptional regulations, we have examined a promoter region between -1742 and -2010. DNase I footprinting assays showed four protected sequences named A, B, C and D. DNA transfections in the T-lymphoid Mo cell line, which constitutively expresses GM-CSF, indicated that the A element, located between -2002 and -1984, has a positive role on transcription. Further characterization by electrophoretic mobility shift assays in the presence of different competitor oligonucleotides showed that this element binds a factor of the NF-kappa B/Rel family.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites
  • Cell Line
  • DNA Footprinting
  • DNA Primers
  • Deoxyribonuclease I
  • Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis*
  • Granulocyte-Macrophage Colony-Stimulating Factor / genetics*
  • HIV Long Terminal Repeat
  • Humans
  • Molecular Sequence Data
  • NF-kappa B / metabolism*
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic*
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-rel
  • Recombinant Proteins / biosynthesis
  • Regulatory Sequences, Nucleic Acid*
  • Transcription Factors
  • Transcription, Genetic
  • Transfection

Substances

  • DNA Primers
  • NF-kappa B
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-rel
  • Recombinant Proteins
  • Transcription Factors
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Deoxyribonuclease I