Abstract
Regulation of the human MHC class I HLA-A11 promoter is governed by a complex array of regulatory elements. One of these elements, shown here to be critical for the transcriptional activity of the promoter, was used to screen a lambda gt11 library and allowed the identification of a cDNA which coded for the zinc finger protein ZFX. ZFX was shown to bind the sequences AGGGCCCCA and AGGCCCCGA, located respectively at positions -271 to -263 and -242 to -234 of the HLA-A11 promoter, with similar affinities through its three C-terminal zinc fingers. ZFX575, a short isoform of ZFX, activates transcription from the HLA-All promoter in a Leydig cell line.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Cell Line
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DNA / chemistry
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DNA / metabolism
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DNA-Binding Proteins / metabolism*
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DNA-Binding Proteins / pharmacology
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Gene Expression Regulation*
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HLA-A Antigens / genetics*
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HLA-A11 Antigen
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HeLa Cells
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Humans
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Kruppel-Like Transcription Factors
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Leydig Cells / metabolism
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Male
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Mice
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Molecular Sequence Data
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Promoter Regions, Genetic*
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Recombinant Proteins / pharmacology
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Transcription Factors
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Transcription, Genetic / drug effects
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Transfection
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Zinc Fingers*
Substances
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DNA-Binding Proteins
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HLA-A Antigens
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HLA-A11 Antigen
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Kruppel-Like Transcription Factors
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Recombinant Proteins
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Transcription Factors
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zinc finger protein, X-linked
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DNA