Transcriptional regulation of the MHC class I HLA-A11 promoter by the zinc finger protein ZFX

Nucleic Acids Res. 1996 May 15;24(10):1928-35. doi: 10.1093/nar/24.10.1928.

Abstract

Regulation of the human MHC class I HLA-A11 promoter is governed by a complex array of regulatory elements. One of these elements, shown here to be critical for the transcriptional activity of the promoter, was used to screen a lambda gt11 library and allowed the identification of a cDNA which coded for the zinc finger protein ZFX. ZFX was shown to bind the sequences AGGGCCCCA and AGGCCCCGA, located respectively at positions -271 to -263 and -242 to -234 of the HLA-A11 promoter, with similar affinities through its three C-terminal zinc fingers. ZFX575, a short isoform of ZFX, activates transcription from the HLA-All promoter in a Leydig cell line.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Line
  • DNA / chemistry
  • DNA / metabolism
  • DNA-Binding Proteins / metabolism*
  • DNA-Binding Proteins / pharmacology
  • Gene Expression Regulation*
  • HLA-A Antigens / genetics*
  • HLA-A11 Antigen
  • HeLa Cells
  • Humans
  • Kruppel-Like Transcription Factors
  • Leydig Cells / metabolism
  • Male
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Recombinant Proteins / pharmacology
  • Transcription Factors
  • Transcription, Genetic / drug effects
  • Transfection
  • Zinc Fingers*

Substances

  • DNA-Binding Proteins
  • HLA-A Antigens
  • HLA-A11 Antigen
  • Kruppel-Like Transcription Factors
  • Recombinant Proteins
  • Transcription Factors
  • zinc finger protein, X-linked
  • DNA