Antroduodenal myoelectric activity was recorded in conscious sheep by electrodes chronically implanted in the muscular wall. Furthermore, plasma immunoreactive (i.r.) motilin, somatostatin and bombesin concentrations were determined by RIA. The intravenous infusion of somatostatin (20 ng/kg/min), bombesin (10 ng/kg/min) or serotonin (5-HT, 4 micrograms/kg/min) for 5 min, induced a duodenal myoelectric activity front followed by a period of quiescence. These duodenal events were concomitant with an antral inhibition. This pattern resembled that observed in a spontaneous migrating myoelectric complex (MMC) in sheep. Bombesin and 5-HT evoked an additional and transient increase in antral activity simultaneously with the duodenal activity front. On the other hand, plasma i.r. motilin levels did not show any fluctuations during spontaneous MMC cycles or after somatostatin, bombesin or 5-HT infusions. Likewise, plasma i.r. bombesin levels remained unchanged during spontaneous MMC or after administration of somatostatin. However, 5-HT -induced duodenal activity fronts were closely associated with a sharp peak in plasma i.r. bombesin. Finally, plasma i.r. somatostatin concentrations rose at the end of spontaneous phase III and peaked in phase I. A similar pattern of somatostatin release in plasma was found while the duodenal activity front and quiescence period developed after either 5-HT or bombesin infusions. These results do not indicate a role for motilin in the control of MMCs in sheep, although a definitive conclusion cannot be drawn until synthetic sheep motilin is available. However, our data suggest that somatostatin and bombesin-like peptides as well as 5-HT, acting in a coordinated manner, could be involved in the regulation of cyclical antroduodenal motor events in sheep.