Structures of new acidic O-specific polysaccharides of the bacterium Proteus mirabilis serogroups O26 and O30

A S Shashkov; F V Toukach; N A Paramonov; A Ziolkowski; S N Senchenkova; W Kaca; Y A Knirel

doi:10.1016/0014-5793(96)00454-1

Structures of new acidic O-specific polysaccharides of the bacterium Proteus mirabilis serogroups O26 and O30

FEBS Lett. 1996 May 20;386(2-3):247-51. doi: 10.1016/0014-5793(96)00454-1.

Authors

A S Shashkov¹, F V Toukach, N A Paramonov, A Ziolkowski, S N Senchenkova, W Kaca, Y A Knirel

Affiliation

¹ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.

PMID: 8647292
DOI: 10.1016/0014-5793(96)00454-1

Abstract

The polysaccharide chains of the lipopolysaccharides of the Proteus mirabilis serogroups O26 and O30 were studied using sugar and methylation analysis and 1H and 13C NMR spectroscopy, including two-dimensional correlation spectroscopy and rotating-frame NOE spectroscopy. The polysaccharides were found to be acidic due to the presence of D-galacturonic acid and its amide with L-lysine in serogroup O26 or D-glucuronic acid in serogroup O30, and the structures of their tetrasaccharide repeating units were established. The O26-specific polysaccharide is structurally and serologically related to the O-specific polysaccharide of P. mirabilis O28, which includes amides of D-GalA with L-lysine and L-serine [Radziejewska-Lebrecht, J. et al. (1995) Eur. J. Biochem. 230, 705-712].

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Conformation
Carbohydrate Sequence
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens / chemistry*
Proteus mirabilis / chemistry*
Proteus mirabilis / classification
Serotyping

Substances

O Antigens