Antibodies specific for the DNA quadruplex [d(CGC G4 GCG)4] isolated from autoimmune mice

Nucleic Acids Symp Ser. 1995:(33):134-6.

Abstract

An autoantibody specific for a DNA quadruplex structure has been isolated and cloned from three-month-old autoimmune "viable motheaten" mice. This antibody (mev-alpha Q1) has been tested extensively in vitro and found to bind specifically and preferentially to the parallel-stranded quadruplex structure formed by the oligonucleotide d(CGC G4 GCG). The anti-quadruplex antibody does not show specific affinity for single-, double-, or triple-stranded oligonuclotides of similar CG-rich sequence motifs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Antinuclear / genetics
  • Antibodies, Antinuclear / isolation & purification*
  • Antibody Specificity
  • Autoimmunity / genetics
  • Base Sequence
  • Cloning, Molecular
  • DNA / chemistry*
  • DNA / genetics
  • DNA / immunology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Molecular Sequence Data

Substances

  • Antibodies, Antinuclear
  • DNA