Abstract
A Mycoplasma hyopneumoniae clone bank was screened with hyperimmune pig serum. One clone exhibited sequence homology to the prokaryotic R2 subunit of ribonucleotide reductase and was expressed as an 11-kDa protein fused to beta-galactosidase. The vaccine potential of the fusion protein was assessed in pig trials. Following experimental challenge with a virulent isolate of M. hyopneumoniae, gross lung pathology (mean Goodwin lung score) of vaccinated animals, irrespective of adjuvant treatment, was significantly reduced compared with that of control unvaccinated pigs (P < 0.05).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Bacterial Vaccines / immunology*
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Base Sequence
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Molecular Sequence Data
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Mycoplasma / enzymology
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Mycoplasma / genetics
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Mycoplasma / immunology*
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Pneumonia, Bacterial / prevention & control
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Pneumonia, Bacterial / veterinary*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Ribonucleotide Reductases / genetics
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Ribonucleotide Reductases / immunology*
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Swine
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Swine Diseases / prevention & control*
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Vaccines, Synthetic / immunology
Substances
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Bacterial Vaccines
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Recombinant Fusion Proteins
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Vaccines, Synthetic
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Ribonucleotide Reductases