Abstract
The role of the insE open reading frame in transposition of IS1 was reexamined by using an insE nonsense mutation that does not alter the amino acid sequence of InsA inhibitor or InsAB transposase. The mutant was active in all strains tested, showing that insE is not essential for formation of cointegrates.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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DNA Transposable Elements / genetics*
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DNA, Bacterial / genetics*
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Enterobacteriaceae / genetics*
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Escherichia coli / genetics
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Mutagenesis, Insertional*
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Open Reading Frames*
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Salmonella typhimurium / genetics
Substances
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DNA Transposable Elements
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DNA, Bacterial