Abstract
The regulatory activity of a 826 bp DNA fragment located upstream of the pTiBo542 TL-DNA gene 6b coding region was analyzed in transgenic tobacco, using beta-glucuronidase (gus) as a reporter gene. The region was shown to drive organ-specific, wound- and auxin-inducible expression of the reporter, the effect being dependent on the type and concentration of auxin.
MeSH terms
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Agrobacterium tumefaciens / genetics*
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Agrobacterium tumefaciens / metabolism
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Base Sequence
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Consensus Sequence
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DNA, Bacterial / genetics*
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DNA, Single-Stranded / genetics
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Gene Expression / drug effects*
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Genes, Bacterial*
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Glucuronidase / biosynthesis
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Indoleacetic Acids / pharmacology*
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Molecular Sequence Data
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Nicotiana / metabolism*
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Organ Specificity
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Plants, Genetically Modified
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Plants, Toxic*
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Recombinant Proteins / biosynthesis
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Sequence Homology, Nucleic Acid
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TATA Box
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Wounds and Injuries
Substances
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DNA, Bacterial
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DNA, Single-Stranded
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Indoleacetic Acids
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Recombinant Proteins
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T-DNA
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Glucuronidase