5'-regulatory region of Agrobacterium tumefaciens T-DNA gene 6b directs organ-specific, wound-inducible and auxin-inducible expression in transgenic tobacco

Plant Mol Biol. 1995 Dec;29(6):1299-304. doi: 10.1007/BF00020470.

Abstract

The regulatory activity of a 826 bp DNA fragment located upstream of the pTiBo542 TL-DNA gene 6b coding region was analyzed in transgenic tobacco, using beta-glucuronidase (gus) as a reporter gene. The region was shown to drive organ-specific, wound- and auxin-inducible expression of the reporter, the effect being dependent on the type and concentration of auxin.

Publication types

  • Comparative Study

MeSH terms

  • Agrobacterium tumefaciens / genetics*
  • Agrobacterium tumefaciens / metabolism
  • Base Sequence
  • Consensus Sequence
  • DNA, Bacterial / genetics*
  • DNA, Single-Stranded / genetics
  • Gene Expression / drug effects*
  • Genes, Bacterial*
  • Glucuronidase / biosynthesis
  • Indoleacetic Acids / pharmacology*
  • Molecular Sequence Data
  • Nicotiana / metabolism*
  • Organ Specificity
  • Plants, Genetically Modified
  • Plants, Toxic*
  • Recombinant Proteins / biosynthesis
  • Sequence Homology, Nucleic Acid
  • TATA Box
  • Wounds and Injuries

Substances

  • DNA, Bacterial
  • DNA, Single-Stranded
  • Indoleacetic Acids
  • Recombinant Proteins
  • T-DNA
  • Glucuronidase

Associated data

  • GENBANK/X89966