Abstract
beta-Galactosidase (beta-gal) expressing vectors are commonly used to standardize the transfection efficiency in transient expression experiments. In the Chinese hamster ovary (CHO) cell line, we transfected beta-gal expressing vectors in combination with different plasmid DNAs. We reported here that the presence of specific DNAs led to statistically significant variations in the beta-gal expression level. Therefore, the measure of beta-gal activity is not necessarily an accurate method to monitor transfection efficiency, and its use to normalize the expression from reporter genes could be questionable.
MeSH terms
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Animals
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Artifacts*
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Avian Sarcoma Viruses / genetics
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CHO Cells / metabolism
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Cricetinae
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Cricetulus
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DNA, Recombinant / genetics
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DNA, Recombinant / pharmacology*
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics
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Evaluation Studies as Topic
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Gene Expression / drug effects*
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Gene Expression Regulation
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Genes, Reporter*
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Genes, Viral
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Genetic Vectors / genetics*
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Promoter Regions, Genetic
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Proto-Oncogene Protein c-fli-1
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Proto-Oncogene Proteins*
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Recombinant Fusion Proteins / biosynthesis*
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Recombinant Fusion Proteins / genetics
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Repetitive Sequences, Nucleic Acid
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Reproducibility of Results
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Simian virus 40 / genetics
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Trans-Activators / biosynthesis
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Trans-Activators / genetics
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Transcriptional Activation*
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Transfection*
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beta-Galactosidase / biosynthesis
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beta-Galactosidase / genetics*
Substances
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DNA, Recombinant
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DNA-Binding Proteins
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Proto-Oncogene Protein c-fli-1
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Proto-Oncogene Proteins
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Recombinant Fusion Proteins
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Trans-Activators
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beta-Galactosidase