Luminol-hemin-H2O2 system was used for chemiluminescent flow injection analysis with immobilized luminol to determine hydrogen peroxide. The flow injection system was simplified. The method is fast and sensitive, and it has a dynamic range of detection from 1.0 x 10(-9) to 5.0 x 10(-4) mol/L with the detection limit of 2.6 x 10(-11) mol/L for H2O2.