Role of Rho in chemoattractant-activated leukocyte adhesion through integrins

Science. 1996 Feb 16;271(5251):981-3. doi: 10.1126/science.271.5251.981.

Abstract

Heterotrimeric guanine nucleotide binding protein (G protein)-linked receptors of the chemoattractant subfamily can trigger adhesion through leukocyte integrins, and in this role they are thought to regulate immune cell-cell interactions and trafficking. In lymphoid cells transfected with formyl peptide or interleukin-8 receptors, agonist stimulation activated nucleotide exchange on the small guanosine triphosphate-binding protein RhoA in seconds. Inactivation of Rho by C3 transferase exoenzyme blocked agonist-induced lymphocyte alpha4beta1 adhesion to vascular cell adhesion molecule-1 and neutrophil beta2 integrin adhesion to fibrinogen. These findings suggest that Rho participates in signaling from chemoattractant receptors to trigger rapid adhesion in leukocytes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, CD / genetics
  • B-Lymphocytes / physiology*
  • Cell Adhesion*
  • Cells, Cultured
  • Chemotactic Factors / pharmacology*
  • GTP-Binding Proteins / metabolism
  • GTP-Binding Proteins / physiology*
  • Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
  • Guanosine Diphosphate / metabolism
  • Integrin alpha4beta1
  • Integrins / physiology*
  • Interleukin-8 / pharmacology
  • Mice
  • Molecular Sequence Data
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Receptors, Formyl Peptide
  • Receptors, Immunologic / genetics
  • Receptors, Interleukin / genetics
  • Receptors, Interleukin-8A
  • Receptors, Lymphocyte Homing / physiology*
  • Receptors, Peptide / genetics
  • Signal Transduction
  • Tetradecanoylphorbol Acetate / pharmacology
  • Transfection
  • Vascular Cell Adhesion Molecule-1 / physiology*
  • rhoA GTP-Binding Protein

Substances

  • Antigens, CD
  • Chemotactic Factors
  • Integrin alpha4beta1
  • Integrins
  • Interleukin-8
  • Receptors, Formyl Peptide
  • Receptors, Immunologic
  • Receptors, Interleukin
  • Receptors, Interleukin-8A
  • Receptors, Lymphocyte Homing
  • Receptors, Peptide
  • Vascular Cell Adhesion Molecule-1
  • Guanosine Diphosphate
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • N-Formylmethionine Leucyl-Phenylalanine
  • GTP-Binding Proteins
  • rhoA GTP-Binding Protein
  • Tetradecanoylphorbol Acetate