A 3.1-Mb YAC contig within the Werner syndrome region, on the short arm of human chromosome 8

M Chaffanet; A Imbert; J Adélaïde; D Le Paslier; M J Wagner; D E Wells; D Birnbaum; M J Pébusque

doi:10.1159/000134164

A 3.1-Mb YAC contig within the Werner syndrome region, on the short arm of human chromosome 8

Cytogenet Cell Genet. 1996;72(1):63-8. doi: 10.1159/000134164.

Authors

M Chaffanet¹, A Imbert, J Adélaïde, D Le Paslier, M J Wagner, D E Wells, D Birnbaum, M J Pébusque

Affiliation

¹ Laboratoire d'Oncologie Moléculaire, U.119 INSERM, Marseille, France.

PMID: 8565638
DOI: 10.1159/000134164

Abstract

The locus for Werner syndrome (WRN) has been localized to human chromosome 8p21 --> p12, close to the anonymous marker D8S339. A 3.1-Mb contig of yeast artificial chromosomes (YACs) was assembled around D8S339. Results from analyses of somatic cell hybrids, FISH, and physical mapping suggest the following loci order: tel-NEFL-D8S131-D8S339-[D8S540/GSR]-D8S124 -D8S259-D8S87-FGFR1-cen. Close physical linkage between D8S540 and GSR was established within a DNA fragment of 200 kb. These two loci are not more than 400 kb from D8S339. In addition, D8S339, D8S540, D8S124, and GSR are within 1.1 Mb. These data establish a primary physical map of the Werner syndrome region and identify useful YAC clones for the isolation of new markers and of the corresponding gene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Mapping
Chromosomes, Artificial, Yeast*
Chromosomes, Human, Pair 8*
Genetic Markers
Humans
In Situ Hybridization, Fluorescence
Restriction Mapping
Werner Syndrome / genetics*

Substances

Genetic Markers