Enhancement of phagocytosis in mouse macrophages by pituitary adenylate cyclase activating polypeptide (PACAP) and related peptides

M Ichinose; M Asai; K Imai; M Sawada

doi:10.1016/0162-3109(95)00025-o

Enhancement of phagocytosis in mouse macrophages by pituitary adenylate cyclase activating polypeptide (PACAP) and related peptides

Immunopharmacology. 1995 Sep;30(3):217-24. doi: 10.1016/0162-3109(95)00025-o.

Authors

M Ichinose¹, M Asai, K Imai, M Sawada

Affiliation

¹ Department of Physiology, Shimane Medical University Izumo, Japan.

PMID: 8557521
DOI: 10.1016/0162-3109(95)00025-o

Abstract

The effects of pituitary adenylate cyclase activating polypeptide (PACAP) and related peptides on phagocytosis of fluorescent latex beads by mouse peritoneal macrophages were examined using flow cytometry (FCM). PACAP38, PACAP27 and vasoactive intestinal peptide (VIP) enhanced phagocytosis in a dose-dependent manner. Relative potencies of related peptides at a concentration of 10(-6) M were PACAP38 > PACAP27 > VIP > secretin > glucagon > (peptide with NH2-terminal histidine and COOH-terminal methionine amide, in short PHM). PACAP(6-38) was as effective as PACAP38. PACAP(6-27) enhanced phagocytosis more effectively than did PACAP27. PACAP(28-38) slightly enhanced phagocytosis. The present result suggest that PACAP38 is one of the mediators that the nervous system uses to modulate the immune system.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Dose-Response Relationship, Drug
Flow Cytometry
Macrophages, Peritoneal / drug effects*
Macrophages, Peritoneal / immunology
Mice
Mice, Inbred BALB C
Neuropeptides / pharmacology*
Peptide Fragments / pharmacology
Phagocytosis / drug effects*
Pituitary Adenylate Cyclase-Activating Polypeptide
Vasoactive Intestinal Peptide / pharmacology

Substances

Adcyap1 protein, mouse
Neuropeptides
Peptide Fragments
Pituitary Adenylate Cyclase-Activating Polypeptide
Vasoactive Intestinal Peptide