Listeriolysin is a potent inducer of the phosphatidylinositol response and lipid mediator generation in human endothelial cells

U Sibelius; F Rose; T Chakraborty; A Darji; J Wehland; S Weiss; W Seeger; F Grimminger

doi:10.1128/iai.64.2.674-676.1996

Listeriolysin is a potent inducer of the phosphatidylinositol response and lipid mediator generation in human endothelial cells

Infect Immun. 1996 Feb;64(2):674-6. doi: 10.1128/iai.64.2.674-676.1996.

Authors

U Sibelius¹, F Rose, T Chakraborty, A Darji, J Wehland, S Weiss, W Seeger, F Grimminger

Affiliation

¹ Department of Internal Medicine, Justus Liebig University, Giessen, Federal Republic of Germany.

Abstract

The impact of Listeria monocytogenes listeriolysin O (LLO) secretion on phosphoinositide metabolism and mediator (platelet-activating factor and prostaglandin I2) generation was investigated in human umbilical vein endothelial cells. Wild-type L. monocytogenes, purified LLO, and an L. innocua strain engineered to secrete LLO all elicited a strong response, whereas mutant strains defective in LLO production were ineffective. Thus, human umbilical vein endothelial cell stimulation by listeriae is linked to production of LLO.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Toxins*
Cells, Cultured
Endothelium, Vascular / drug effects*
Endothelium, Vascular / metabolism
Epoprostenol / biosynthesis*
Heat-Shock Proteins / pharmacology*
Hemolysin Proteins / pharmacology*
Humans
L-Lactate Dehydrogenase / metabolism
Phosphatidylinositols / biosynthesis*
Platelet Activating Factor / biosynthesis*

Substances

Bacterial Toxins
Heat-Shock Proteins
Hemolysin Proteins
Phosphatidylinositols
Platelet Activating Factor
Epoprostenol
L-Lactate Dehydrogenase
hlyA protein, Listeria monocytogenes