Processing, purification, and kinetic characterization of the Gag-Pol encoded retroviral proteinase of myeloblastosis associated virus expressed in E. coli

Adv Exp Med Biol. 1995:362:485-8. doi: 10.1007/978-1-4615-1871-6_64.

Authors

J Brynda¹, M Fábry, P J Tichý, M Horejsí, J Sedlácek

Affiliation

¹ Department of Gene Manipulation, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

PMID: 8540362
DOI: 10.1007/978-1-4615-1871-6_64

No abstract available

Publication types

Comparative Study

MeSH terms

Amino Acid Sequence
Aspartic Acid Endopeptidases / biosynthesis
Aspartic Acid Endopeptidases / isolation & purification
Aspartic Acid Endopeptidases / metabolism*
Avian Myeloblastosis Virus / enzymology*
Avian Myeloblastosis Virus / genetics
Avian Sarcoma Viruses / enzymology*
Avian Sarcoma Viruses / genetics
Cloning, Molecular / methods
Electrophoresis, Polyacrylamide Gel
Endopeptidases / biosynthesis
Endopeptidases / isolation & purification
Endopeptidases / metabolism*
Escherichia coli
Fusion Proteins, gag-pol / biosynthesis
Fusion Proteins, gag-pol / isolation & purification
Fusion Proteins, gag-pol / metabolism*
Genes, gag*
Genes, pol*
Kinetics
Molecular Sequence Data
Terminator Regions, Genetic

Substances

Fusion Proteins, gag-pol
Endopeptidases
Aspartic Acid Endopeptidases
Rous sarcoma virus protease
protease p15