NMR investigation of the 6-deoxy-L-talose-containing O45, O45-related (O45rel), and O66 polysaccharides of Escherichia coli

B Jann; A Shashkov; V Torgov; H Kochanowski; G Seltmann; K Jann

doi:10.1016/0008-6215(95)00243-6

NMR investigation of the 6-deoxy-L-talose-containing O45, O45-related (O45rel), and O66 polysaccharides of Escherichia coli

Carbohydr Res. 1995 Nov 30;278(1):155-65. doi: 10.1016/0008-6215(95)00243-6.

Authors

B Jann¹, A Shashkov, V Torgov, H Kochanowski, G Seltmann, K Jann

Affiliation

¹ Max-Planck-Institut für Immunobiologie, Freiburg, Germany.

PMID: 8536267
DOI: 10.1016/0008-6215(95)00243-6

Abstract

The structures of the 6-deoxytalose-containing O-specific polysaccharides from the O45 antigen, an O45-related antigen (O45rel), and the O66 antigen (lipopolysaccharides, LPSs) of Escherichia coli were elucidated by chemical characterization and by one- and two-dimensional 1H and 13C NMR spectroscopy. The O45 and O45-related polysaccharides have the following general structure: [formula: see text] For the O45 antigen, X is alpha-D-FucpNAc and for the O45-related antigen, X is beta-D-GlcpNAc. The structure of the O66 polysaccharide is [formula: see text]

MeSH terms

Carbohydrate Conformation
Carbohydrate Sequence
Deoxy Sugars / chemistry*
Deoxy Sugars / isolation & purification
Escherichia coli / chemistry*
Escherichia coli / classification
Hexoses*
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens / chemistry*
O Antigens / isolation & purification
Serotyping

Substances

Deoxy Sugars
Hexoses
O Antigens
6-deoxytalose