Observations on the effect of the diluent used for diluting challenge toxin in the Clostridium botulinum potency test

Biologicals. 1995 Sep;23(3):249-51. doi: 10.1006/biol.1995.0041.

Abstract

Clostridium botulinum vaccines were assayed for potency according to the method described in the European Pharmacopoeia. This method uses 20 white mice which are vaccinated with a dilution of vaccine in 0.9% saline. Twenty-one days later the vaccinated mice and ten unvaccinated control mice are challenged with C. botulinum toxin and observed for 7 days. In this study the challenge toxin was diluted in two separate diluents, 0.9% saline and peptone broth. More reproducible results were achieved with peptone broth as the diluent.

MeSH terms

  • Animals
  • Bacterial Vaccines / chemistry
  • Bacterial Vaccines / immunology*
  • Botulinum Toxins / chemistry
  • Botulinum Toxins / immunology*
  • Clostridium botulinum / immunology*
  • Female
  • Indicator Dilution Techniques
  • Mice
  • Peptones
  • Reproducibility of Results
  • Sodium Chloride
  • Solvents
  • Vaccination

Substances

  • Bacterial Vaccines
  • Peptones
  • Solvents
  • Sodium Chloride
  • Botulinum Toxins