Purification and characterization of (S)-1,3-butanediol dehydrogenase from Candida parapsilosis

Biosci Biotechnol Biochem. 1995 Sep;59(9):1769-70. doi: 10.1271/bbb.59.1769.

Abstract

(S)-1,3-Butanediol (BDO) oxidizing enzyme was purified from Candida parapsilosis IFO 1396, which could produce (R)-1,3-BDO from the racemate. The purified enzyme was an NAD(+)-dependent secondary alcohol dehydrogenase that oxidized (S)-1,3-BDO to 4-hydroxy-2-butanone stereo-specifically.

MeSH terms

  • Alcohol Oxidoreductases / chemistry*
  • Alcohol Oxidoreductases / drug effects
  • Alcohol Oxidoreductases / isolation & purification*
  • Butylene Glycols / metabolism
  • Candida / enzymology*
  • Electrophoresis, Polyacrylamide Gel / methods
  • Molecular Weight
  • NAD / metabolism
  • Oxidation-Reduction
  • Phenanthrolines / pharmacology
  • Spectrophotometry
  • Substrate Specificity

Substances

  • Butylene Glycols
  • Phenanthrolines
  • NAD
  • 1,3-butylene glycol
  • Alcohol Oxidoreductases
  • butanediol dehydrogenase
  • 1,10-phenanthroline