Abstract
(S)-1,3-Butanediol (BDO) oxidizing enzyme was purified from Candida parapsilosis IFO 1396, which could produce (R)-1,3-BDO from the racemate. The purified enzyme was an NAD(+)-dependent secondary alcohol dehydrogenase that oxidized (S)-1,3-BDO to 4-hydroxy-2-butanone stereo-specifically.
MeSH terms
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Alcohol Oxidoreductases / chemistry*
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Alcohol Oxidoreductases / drug effects
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Alcohol Oxidoreductases / isolation & purification*
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Butylene Glycols / metabolism
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Candida / enzymology*
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Electrophoresis, Polyacrylamide Gel / methods
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Molecular Weight
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NAD / metabolism
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Oxidation-Reduction
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Phenanthrolines / pharmacology
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Spectrophotometry
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Substrate Specificity
Substances
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Butylene Glycols
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Phenanthrolines
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NAD
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1,3-butylene glycol
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Alcohol Oxidoreductases
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butanediol dehydrogenase
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1,10-phenanthroline