Abstract
An endo-beta-1,4-D-galactanase was purified 1735-fold from a commercial enzyme preparation of Aspergillus niger. The endogalactanase was homogeneous by SDS-PAGE, having an apparent molecular weight of 32,000. It specifically hydrolyzed beta-1,4-D-galactan, and is shown to be capable of releasing galactosyl oligomers and galactose from the soybean pectic polysaccharides.
MeSH terms
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Aspergillus niger / enzymology*
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Chromatography, High Pressure Liquid
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Electrophoresis, Polyacrylamide Gel / methods
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Glycoside Hydrolases*
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Hydrogen-Ion Concentration
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Polysaccharides / chemistry
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Polysaccharides / metabolism
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Substrate Specificity
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Temperature
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beta-Galactosidase / chemistry*
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beta-Galactosidase / isolation & purification
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beta-Galactosidase / metabolism*
Substances
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Polysaccharides
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Glycoside Hydrolases
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beta-Galactosidase
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arabinogalactan endo-1,4-beta-galactosidase