Purification and properties of endo-beta-1,4-D-galactanase from Aspergillus niger

Biosci Biotechnol Biochem. 1995 Sep;59(9):1742-4. doi: 10.1271/bbb.59.1742.

Abstract

An endo-beta-1,4-D-galactanase was purified 1735-fold from a commercial enzyme preparation of Aspergillus niger. The endogalactanase was homogeneous by SDS-PAGE, having an apparent molecular weight of 32,000. It specifically hydrolyzed beta-1,4-D-galactan, and is shown to be capable of releasing galactosyl oligomers and galactose from the soybean pectic polysaccharides.

MeSH terms

  • Aspergillus niger / enzymology*
  • Chromatography, High Pressure Liquid
  • Electrophoresis, Polyacrylamide Gel / methods
  • Glycoside Hydrolases*
  • Hydrogen-Ion Concentration
  • Polysaccharides / chemistry
  • Polysaccharides / metabolism
  • Substrate Specificity
  • Temperature
  • beta-Galactosidase / chemistry*
  • beta-Galactosidase / isolation & purification
  • beta-Galactosidase / metabolism*

Substances

  • Polysaccharides
  • Glycoside Hydrolases
  • beta-Galactosidase
  • arabinogalactan endo-1,4-beta-galactosidase