The multiplicity of enzymatic DNA reduction: a new purification procedure for a DHA reducing protein from potato tubers

Boll Soc Ital Biol Sper. 1995 May-Jun;71(5-6):163-70.

Abstract

The multiplicity of DHA reducing proteins has been observed by means of a native-PAGE technique in several plant species, thus demonstrating, in accordance with recent literature, that several differently evolved proteins are likely to perform DHA reduction. Moreover, a research strategy coupling the use of native-PAGE with chromatographic separation procedure, tentatively performed in Solanum tuberosum, proved to be a useful tool for the separation and partial identification of the proteins involved in DHA reduction.

Publication types

  • Comparative Study

MeSH terms

  • Chromatography, Ion Exchange
  • Dehydroascorbic Acid / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Oxidation-Reduction
  • Oxidoreductases / isolation & purification*
  • Plant Proteins / isolation & purification*
  • Plants, Edible / enzymology
  • Solanum tuberosum / enzymology*

Substances

  • Plant Proteins
  • Oxidoreductases
  • glutathione dehydrogenase (ascorbate)
  • Dehydroascorbic Acid