[Drosophila beta-heterochromatin: molecular organization and function. Cloning and molecular biological analysis of the lambda 20 DNA fragment from Drosophila melanogaster beta-heterochromatin]

S I Baĭborodin; E M Baricheva; S S Bogachev; I V Borisevich; O V Strots; M A Filippova; I V Sharakhov; A G Shilov

[Drosophila beta-heterochromatin: molecular organization and function. Cloning and molecular biological analysis of the lambda 20 DNA fragment from Drosophila melanogaster beta-heterochromatin]

Genetika. 1993 Mar;29(3):403-16.

[Article in Russian]

Authors

S I Baĭborodin, E M Baricheva, S S Bogachev, I V Borisevich, O V Strots, M A Filippova, I V Sharakhov, A G Shilov

PMID: 8486265

Abstract

To isolate the DNA sequences specific for the pericentric heterochromatin of Drosophila we used two CREST-autoimmune sera which bind in the Western-blot analysis the nuclear antigens of 30 kDa, 43 kDa and 45 kDa molecular weight. Cloning of the DNA fragments associated with these CREST-specific proteins of Drosophila resulted in obtaining 8 clones. One of them, lambda 20, hybridized mainly to the chromomcenter of polytene chromosomes. The further analysis indicated that the lambda 20 DNA might belong to the proximal beta-heterochromatin of the polytene chromosomes of D. melanogaster.

Publication types

English Abstract

MeSH terms

Animals
Antibodies / immunology
Chromosomes
Cloning, Molecular
Cross Reactions
DNA / chemistry
DNA / genetics*
Drosophila melanogaster / genetics*
Heterochromatin*
Humans
Immune Sera
In Situ Hybridization
Microscopy, Electron
Mitosis
Nuclear Proteins / immunology
Nuclear Proteins / ultrastructure

Substances

Antibodies
Heterochromatin
Immune Sera
Nuclear Proteins
DNA