Structural determination of the capsular polysaccharide produced by Klebsiella pneumoniae serotype K40. NMR studies of the oligosaccharide obtained upon depolymerisation of the polysaccharide with a bacteriophage-associated endoglycanase

Eur J Biochem. 1993 Apr 1;213(1):445-53. doi: 10.1111/j.1432-1033.1993.tb17780.x.

Abstract

The Klebsiella pneumoniae K40 capsular polysaccharide has been isolated and investigated by use of methylation analysis, specific degradations and NMR spectroscopy. The polysaccharide was depolymerised by a bacteriophage-associated endogalactosidase, and the resulting oligosaccharide was characterised by one-dimensional and two-dimensional NMR spectroscopy and direct chemical ionisation MS. The repeating unit of the K40 capsular polysaccharide was shown to be a linear hexasaccharide with the composition-->3)- alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->4)-alpha-D-GlcpA++ +-(1-->2-)- alpha-D-Manp-(1-->2)-alpha-D-Manp-(1-->3)-alpha-D-Galp-(1--> (Rha, rhamnose).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / enzymology
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Cellulase / metabolism
  • Klebsiella pneumoniae / metabolism*
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Methylation
  • Molecular Sequence Data
  • Oligosaccharides / chemistry
  • Polymers / chemistry
  • Polysaccharides, Bacterial / biosynthesis
  • Polysaccharides, Bacterial / chemistry*
  • Sugar Alcohols / chemistry

Substances

  • Oligosaccharides
  • Polymers
  • Polysaccharides, Bacterial
  • Sugar Alcohols
  • Cellulase