Deletion of IRF-1, mapping to chromosome 5q31.1, in human leukemia and preleukemic myelodysplasia

Science. 1993 Feb 12;259(5097):968-71. doi: 10.1126/science.8438156.

Abstract

One of the most frequent cytogenetic abnormalities in human leukemia and myelodysplasia is an interstitial deletion within chromosome 5q. A tumor suppressor gene has been hypothesized to lie in 5q31, the smallest commonly deleted region. IRF-1, a gene whose product manifests anti-oncogenic activity, was mapped to 5q31.1. IRF-1 lies between IL-5 and CDC25C and is centromeric to IL-3 and GM-CSF. Among these genes, only IRF-1 was consistently deleted at one or both alleles in 13 cases of leukemia or myelodysplasia with aberrations of 5q31. Inactivating rearrangements of one IRF-1 allele, accompanied by deletion of the second allele, were also identified in one case of acute leukemia. Thus, IRF-1 may be a critically deleted gene in human leukemia and myelodysplasia.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 5*
  • DNA Probes
  • DNA-Binding Proteins / genetics*
  • Gene Deletion*
  • Gene Rearrangement
  • Genes, Tumor Suppressor*
  • Humans
  • In Situ Hybridization
  • Interferon Regulatory Factor-1
  • Leukemia / genetics*
  • Molecular Sequence Data
  • Myelodysplastic Syndromes / genetics*
  • Phosphoproteins / genetics*
  • Polymerase Chain Reaction

Substances

  • DNA Probes
  • DNA-Binding Proteins
  • IRF1 protein, human
  • Interferon Regulatory Factor-1
  • Phosphoproteins