Predicting the effects of photodynamic therapy (PDT) and Nd: YAG laser coagulation requires knowledge of the light distribution in tumor and surrounding tissue. Therefore, absorption and scattering coefficients and the average cosine of the scattering angle (the anisotropy factor) were measured in rat liver and tumor at 632.8 and 1064 nm. A syngenic colon adenocarcinoma CC531 was implanted subcutaneously in two groups of 7 Wag/Rij rats. In one group Photofrin was administered 5 mg/kg intravenously 48 h before determination of optical properties. Two months after inoculation, samples were taken from tumor and liver and optical properties determined using indirect methods with two integrating spheres. The absorption coefficient was larger in liver than in tumor at 632.8 nm (P < 0.0005), whereas the scattering coefficient was larger in tumor than in liver at 1,064 nm (P < 0.05). Addition of Photofrin increased the scattering coefficient in liver and in tumor at both wavelengths (P < 0.025) and decreased the anisotropy in tumor (P < 0.025), suggesting that for modelling the dosimetry of PDT the optical properties of photosensitized tissue should be used.