Abstract
In analyzing the region of the Saccharopolyspora erythraea chromosome responsible for the biosynthesis of the macrolide antibiotic erythromycin, we identified a gene, designated eryK, located about 50 kb downstream of the erythromycin resistance gene, ermE. eryK encodes a 44-kDa protein which, on the basis of comparative analysis, belongs to the P450 monooxygenase family. An S. erythraea strain disrupted in eryK no longer produced erythromycin A but accumulated the B and D forms of the antibiotic, indicating that eryK is responsible for the C-12 hydroxylation of the macrolactone ring, one of the last steps in erythromycin biosynthesis.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins*
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Base Sequence
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Chromosome Mapping
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Consensus Sequence
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Cytochrome P-450 Enzyme System / classification
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Cytochrome P-450 Enzyme System / genetics*
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Erythromycin / biosynthesis*
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Genes, Bacterial / genetics*
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Hydroxylation
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Mixed Function Oxygenases / genetics*
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Molecular Sequence Data
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Mutagenesis, Insertional
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Oxygenases / classification
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Oxygenases / genetics*
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Saccharopolyspora / enzymology
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Saccharopolyspora / genetics*
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Sequence Homology, Amino Acid
Substances
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Bacterial Proteins
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Erythromycin
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Cytochrome P-450 Enzyme System
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Mixed Function Oxygenases
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Oxygenases
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EryK protein, Saccharopolyspora erythraea