Abstract
Amphotericin B (AmB) in aqueous solution becomes less aggregated as temperature is increased as measured by CD spectroscopy. Concomitantly, AmB's ability to induce K+ leakage from cholesterol-containing large unilamellar vesicles (LUV) decreases 4-fold per 10 degrees C increase. In contrast, ergosterol-containing vesicles show no clear trend in their temperature sensitivity to AmB action. These data strongly support a model of AmB action where drug aggregates and not monomers are the major channel-inducing species against cholesterol- but not ergosterol-containing membranes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amphotericin B / administration & dosage
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Amphotericin B / chemistry*
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Amphotericin B / toxicity
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Arylsulfonates
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Cell Membrane / chemistry*
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Cell Membrane / drug effects
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Cholesterol / chemistry
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Circular Dichroism
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Ergosterol / chemistry
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Hydrogen-Ion Concentration
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Lipid Bilayers / chemistry*
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Liposomes / chemistry
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Models, Biological
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Potassium / chemistry
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Temperature*
Substances
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Arylsulfonates
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Lipid Bilayers
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Liposomes
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Amphotericin B
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Cholesterol
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pyranine
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Potassium
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Ergosterol