Abstract
TR3 orphan receptor is a human homologue of the mouse nur77, N10 and rat NGFI-B, TIS1 genes which may represent an early response gene involved in the control of cell proliferation. We have studied potential target genes for TR3 orphan receptor using the DNA-binding domain replacement method. We found that mouse mammary tumor virus long terminal repeat-linked chloramphenicol acetyltransferase expression can be activated in transfected cells by a chimeric androgen receptor/TR3 orphan receptor/androgen receptor construct (AR/TR3/AR) in the presence of androgen. By deletion analysis, a region with 20 nucleotides in length between positions -1178 and -1159 of the mouse mammary tumor virus long terminal repeat was confirmed as a potential TR3 orphan receptor response element. These results suggest that feasibility of using the DNA-binding domain replacement method to detect target sequences of orphan receptors.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Androgens / pharmacology
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Base Sequence
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Chloramphenicol O-Acetyltransferase / biosynthesis
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Chloramphenicol O-Acetyltransferase / metabolism
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism*
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Enzyme Induction
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Humans
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Male
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Mammary Tumor Virus, Mouse / genetics*
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Nuclear Receptor Subfamily 4, Group A, Member 1
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Oligodeoxyribonucleotides
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Prostatic Neoplasms
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Receptors, Androgen / genetics
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Receptors, Androgen / metabolism
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Receptors, Steroid*
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Receptors, Thyroid Hormone*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / metabolism
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Repetitive Sequences, Nucleic Acid*
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Transcription Factors / genetics*
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Transcription Factors / metabolism*
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Transfection
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Tumor Cells, Cultured
Substances
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Androgens
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DNA-Binding Proteins
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NR4A1 protein, human
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Nr4a1 protein, mouse
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Nr4a1 protein, rat
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Nuclear Receptor Subfamily 4, Group A, Member 1
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Oligodeoxyribonucleotides
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Receptors, Androgen
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Receptors, Steroid
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Receptors, Thyroid Hormone
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Recombinant Fusion Proteins
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Transcription Factors
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Chloramphenicol O-Acetyltransferase