Abstract
Using reverse transcription-polymerase chain reaction (RT-PCR) and degenerate oligonucleotide primers, we amplified novel members of two different subfamily of G-protein coupled receptor (GCR) superfamily from bovine taste tissue. Type A receptor clones composed of multiple cDNA clones had significant similarity with putative olfactory receptor subfamily, while a single type B clone had significant similarity with peptide receptor subfamily. Physiological functions of these receptors in taste cells are discussed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cattle
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DNA
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GTP-Binding Proteins / biosynthesis
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GTP-Binding Proteins / metabolism*
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Gene Expression
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Gene Library
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Mice
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Molecular Sequence Data
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Multigene Family
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Oligodeoxyribonucleotides
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Polymerase Chain Reaction / methods
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RNA, Messenger / biosynthesis
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Rats
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Receptors, Cell Surface / biosynthesis*
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Sensory Receptor Cells / metabolism*
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Sequence Homology, Amino Acid
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Taste / physiology*
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Tongue / metabolism*
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Tongue / physiology
Substances
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Oligodeoxyribonucleotides
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RNA, Messenger
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Receptors, Cell Surface
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DNA
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GTP-Binding Proteins
Associated data
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GENBANK/L16871
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GENBANK/L16872
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GENBANK/L16873
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GENBANK/L16874
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GENBANK/L16875
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GENBANK/L16876
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GENBANK/L16877
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GENBANK/L16878
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GENBANK/S63847
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GENBANK/S63848